然而，超高速摄影机的发展, 加上光学标测系统的演进, 研究人员利用电压敏感染料光学记录膜电位技术 ( Optical Mapping ) 已成为一种较理想的新技术.  

相较于传统的微电极 ( Micro-electrode )、膜片钳技术 ( Patch Clamp ), 脑片膜电位 ( Brain Slice ), 测钙技术 ( Calcium imaging ) 相比,它不仅可以多位点、同步测量膜电位的变化,更可以清楚的标测出细胞群神经电气活动的传导特性,为研究较小细胞和纤细的神经突起、心律失常等生理活动提供了可能方向.

此外, 利用此方法, 可以避免外部令人困扰的电磁场干扰,因此已成为研究心脏电除颤等机理较的手段. SciMedia 更开创使用了组同步的高速摄影机, 可以有效应用于多光谱的光学标测, 广泛应用在离子通道, 荧光离子流应用.  

不仅可以多位点同步记录、无损地记录心肌细胞群的膜电位变化，测量细胞与细胞间的电传导，而且可以记录全心脏的电活动，并转化为细胞动作电位以及除极复极等时图、等势图和动作电位时程图等彩色的图像显示。

此外，在心肌细胞膜电位动态光学成像可以使心脏生理信息检测三维图像可视化。 也可以用于“定量神经电生理学和脑功能成像等领域的研究，为进一步研究人体生理、病理和临床医学诊断, 器官移植, 人造器官, 提供了一种创新方法  

应用范例 : 在无光损伤 ( Photo damaged ) 和 染料的药毒性副作用下, 采用电压敏感染料 ( RH155 ) 多位点光学成像系统可进行多个和成群前庭神经节细胞 (vestibular ganglion cells, VGCs ) VGCs膜电位变化电生理特性.

Di-4-ANEPPS and Di-8-ANEPPS   电压敏感染料光谱

The ANEP (AminoNaphthylEthenylPyridinium) dyes developed by Leslie Loew and colleagues are among the most sensitive of the fast-response probes. Di-4-ANEPPS  and di-8-ANEPPS exhibit fairly uniform 10% per 100 mV changes in fluorescence intensity in a variety of tissue, cell and model membrane systems. The millisecond-range temporal characteristics of the ANEP dyes compensate for this modest response amplitude . Di-4-ANEPPS is internalized in the cell rather rapidly, precluding its use in all but very short-term experiments, whereas di-8-ANEPPS is better retained in the outer leaflet of the plasma membrane. In addition, although both ANEP dyes exhibit good photostability and low toxicity, di-8-ANEPPS is reported to be slightly more photostable and significantly less phototoxic than di-4-ANEPPS ( ... also see RH dye )

di-8-ANEPPS  spectral ....

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