ASI  其他相关产品与技术
相关配备
Microscope 显微镜
Motorized Stage 电控载物台
Fluorescence Filter 荧光滤镜
Filter Wheel 电控滤镜转盘
VYSIS filters VYSIS 荧光滤镜
Cooled CCD Camera 冷却数字摄影机
C-Mount CCD Adapter 摄影转接头

使用 ASI 产品所发表的研究学术报告 ( 摘录部分文献 )  

  1. C. Rothmann, Z. Malik, A.M. Cohen. (1998) Spectrally resolved imaging of Cabot rings and Howell-Jolly bodies. Photochem. Photobiol. 68: 584-7.
  2. H. Tsurui, H. Nishimura, S. Hattori, S. Hirose, K. Okumura, T. Shirai. (2000) Seven-color fluorescence imaging of tissue samples based on fourier spectroscopy and singular value decomposition. Journal of Histochemistry and Cytochemistry, Vol. 48 (5): 653-662.
  3. I. Barshack, J. Kopolovic, Z. Malik, C. Rothmann. (1999) Spectral morphometric characterization of breast carcinoma cells. Br. J. Cancer, 79: 1613-1619.
  4. Lior Greenbaum, Chana Rothmann, Ronit Lavie and Zvi Malik. (2000) Green Fluorescent Protein Photobleaching: a Model for Protein Damage by Endogenous and Exogenous Singlet Oxygen. Biol. Chem., Vol. 381, pp. 1251 – 1258, December 2000.
  5. Macville MV, Speel EJ, Katzir N, Garini Y, Soenksen D, McNamara G, de Wilde PC, Hanselaar AG, Hopman AH, Ried T. (2001) Spectral imaging of multi-color chromogenic dyes in pathological specimens. Anal Cell Pathol 2001, 22(3): 133-142.
  6. M.L. Miller, A. Andriga, J. Elliott, K. Conwell II, K. Dixon, M.P. Carty. (1998) The morphological and spectral phenotype of apoptosis in HeLa cells varies following exposure to UV-C and the addition of inhibitors of ICE and CPP32. Cell Prolif., 31: 17-33.
  7. Andersson, J. et al, The Complexity of KIT Gene Mutations and Chromosome Rearrangements and Their Clinical Correlation in Gastrointestinal Stromal (Pacemaker Cell) Tumors. Am J Pathol, 2002. 160(1): p. 15-22).

 

SPECTRAL-KARYOTYPING REFRENCES    

A. HEMATOLOGICAL MALINANCIES

  1. E.W. Fleischman, S. Reshmi, O.I. Sokova, O.P. Kirichenko, L.N. Konstantinova, O.E. Kulagina, M.A. Frenkel, J.D. Rowley. (1999) Increased karyotype precision using fluorescence in situ hybridization and spectral karyotyping in patients with myeloid malignancies. Cancer Genet. Cytogenet. 108: 166-70.

We studied seven patients with various malignant hematologic disorders using fluorescence in situ hybridization (FISH) and one of these patients with spectral karyotyping (SKY). With appropriate probes, the t(8;21) and inv(16) were confirmed in two patients and the karyotypic precision was increased in five others using FISH and SKY. Two of three patients with 12p rearrangements had a deletion of one TEL allele. Thus, these newer techniques are an important adjunct to accurate chromosome analysis in malignancy.

  1. E. Hilgenfeld, H. Padilla-Nash, E. Schr?ck, T. Ried. (1999) Analysis of B-cell neoplasias by spectral karyotyping. Curr. Top. Microbiol. Immunol. 246: 169-174.

The potential of SKY is exemplified by the fact that in our experience, 70% of the cases analyzed resulted in karyotypes where the majority of aberrations were either refined or new aberrations were detected when compared to their G-banding karyotypes. This also applies to the analysis of B-cell neoplasias. In hematologic malignancies, especially acute leukemias, specific chromosomal aberrations are of etiologic as well as diagnostic and prognostic importance. The identification of new recurrent chromosomal aberrations could therefore lead to a better characterization of disease entities or subgroups in ALL and NHL and further improve diagnosis, treatment stratification and ultimately prognosis.

  1. N. Kakazu, M. Taniwaki, S. Horiike, K. Nishida, T. Tatekawa, M. Nagai, T. Takahashi, T. Akaogi, J. Inazawa , M. Ohki, T. Abe. (1999) Combined spectral karyotyping and DAPI banding analysis of chromosome abnormalities in myelodysplastic syndrome. Genes Chromosomes Cancer 26 (4: 336-345.

Using Spectral Karyotyping (SKY) we have studied chromosome abnormalities found in 9 patients with primary melodysplastic syndrome (MDS), 2 with therapy related MDS, 3 with overt leukemia development from MDS, and one MDS cell line. SKY allows to identify structure of derivative chromosome in 8 pateints, marker chromosome in 5, and subtle chromosomal translocations in 4.  Using dual karyotype analysis by combining SKY and DAPI-banding analysis, we have successfully identified both the origin of rearranged chromosomal materials and breakpoints in a single analysis.

  1. B. Mohr, M. Bornhäuser, C. Thiede, U. Schäkel, M. Schaich, T. Illmer, U. Pascheberg, G. Ehninger. (2000) Comparison of spectral karyotyping and conventional cytogenetics in 39 patients with acute myeloid leukemia and myelodysplastic syndrome. Leukemia 14: 1031-1038.

Spectral karyotyping (SKY) was performed in patient with acute myeloid leukemia (AML; n=25), secondary AML (s-AML; n=7), myelodysplastic syndrome (MDS; n=6) and sMDS (n=1). SKY was helpful for the delination of marker chromosomes and additional material. In addition, SKY could distinguish between partial and total monosomies or real existing and apparent deletions. In two patient a hidden translocation t(7;14) could be revealed with SKY. This translocation was not detected by conventional cytogenetics.

  1.  F.F. Zhang, J.L. Murata-Collins, P. Gaytan, S.J. Forman, K.J. Kopecky, C.L. Willman, F.R. Appelbaum, M.L. Slovak. (2000) Twenty-four-color spectral karyotyping reveals chromosome aberrations in cytogenetically normal acute myeloid leukemia . Genes, Chromosomes and Cancer 28: 318-328.

In the test population of 28 cytogenetically normal acute myeloid leukemia (AML) samples, spectral karyotyping identified previously undetected cytogenetic aberrations in two cases (7%) of karyotypic normal AML. Both abnormalities: a cryptic 11q23 and a minor monosomy 7 clone are considered to confer a poor prognosis.

  1. H. Zattara-Cannoni, H. Dufour, H. Lepidi, C. Chatel, F. Grisoli, A.M. Vagner-Capodano. (1998) Hidden chromosome abnormalities in a primary central nervous system lymphoma detected by multicolor spectral karyotyping. Cancer Genet. Cytogenet. 107: 98-101.

We report here a case of primary central nervous system lymphoma in which chromosomal rearrangements and marker chromosomes not identified by a routine cytogenetic technique were clarified by SKY. This shows the value of the SKY technique in the cytogenetic diagnosis of tumors.

  1. H.F. Mark, Y. Gray, Y. Mark, J. Khorsand, W. Sikov. (1999) A multimodal approach in the diagnosis of patients with hematopoietic disorders. Cancer Genet. Cytogenet. 109: 14-20.

The investigational technique of fluorescence in situ hybridization (FISH), using both painting and alpha-satellite probes, was used as an adjunct to conventional cytogenetics to further delineate the nature of the chromosome abnormalities observed in the GTG-banded studies. Confirmatory studies utilizing the new technique of spectral karyotyping (SKY) were also carried out. Thus, the multimodal approach of hematopathology, GTG-banding, chromosome morphometry, FISH, and SKY can be very useful for delineating complex cytogenetic cases.

  1. P.H. Rao, J.C. Cigudosa, Y. Ning, M.J. Calasanz, S. Iida, S. Tagawa, J. Michaeli, B. Klein, R. Dalla-Favera, S.C. Jhanwar, T. Ried, R.S. Chaganti. (1998) Multicolor spectral karyotyping identifies new recurring breakpoints and translocations in multiple myeloma. Blood 92: 1743-1748.

SKY was used to analyze a panel of nine bone marrow biopsy samplesform eight patients and 10 tumor celllnesderived from MM patients. Using this method it was possible to define all chromosomal reareangemts and identify all of the clonal marker chromosomes in th tumor cells. It was also possible to identify several novel recurring

  1. J.D. Rowley, S. Reshmi, K. Carlson, D. Roulston. (1999) Spectral Karyotype Analysis of T-Cell Acute Leukemia. Blood 93: 2038-2042.

In 15 cases of T-cell acute lymphoblastic leukemia, SKY clarified the chromosome rearrangements in 3 cases and confirm them in 11 others. Thus the use of SKY substantially improves the precision of karyotype analysis of malignant cells, which in turn leads to a more accurate assessment of the genotypic abnormalities in those cells..

  1. J.R. Sawyer, J.L. Lukacs, N. Munshi, K.R. Desikan, S. Singhal, J. Mehta, D. Siegel, J. Shaughnessy, B. Barlogie. (1998) Identification of New Nonrandom Translocations in Multiple Myeloma With Multicolor Spectral Karyotyping. Blood 92: 4269-4278.

Multicolor spectral karyotyping (SKY) was performed on bone marrow samples from 50 patients with multiple myeloma (MM) in anticipation of discovering new previously unidentified translocations. All samples showed complex karyotypes with chromosome aberrations which, in most cases, were not fully characterized by G-banding. The SKY technique was able to refine the designations of over 156 aberrations not fully characterized by G-banding in this study and resolved additional chromosome aberrations in every patient studied except two.  Therefore, the SKY technique provides a useful adjunct to routine G-banding and fluorescence in situ hybridization studies in the cytogenetic analysis of MM.

  1. B. Stark, M. Jeison, R. Gobuzov, S. Finkelshtein, S. Ash, G. Avrahami, I. J. Cohen, J. Stein, I. Yaniv, R. Zaizov, I. Bar-Am. (2000) Apparently Unrelated Clones Shown by Spectral Karyotyping to Represent Clonal Evolution of Cryptic t(10;11)(p13;q23) in a Patient with Acute Monoblastic Leukemia. Cancer Genet Cytogenet 120:105–110.

The accurate genetic classification of acute leukemia is of the utmost clinical importance for treatment stratification. In the present study, we report on a young girl with aggressive acute monoblastic leukemia (AML) (M5b) with skin, lymph node, and bone marrow involvement, in whom cytogenetic analysis revealed three clones with different secondary chromosomal changes. Using the spectral karyotyping (SKY) technique, we found that all three clones originated from a common clone that harbored the hidden primary t(10;11)(p13;q23) or its derivatives, suggesting clonal evolution.  In conclusion, the detection of the very poor prognostic t(10;11) aberration in AML, was possible by complementing the traditional cytogenetic analysis with SKY and FISH.

  1. T.S. Wan, S.K. Ma, G.C. Chan, L.M. Ching, S.Y. Ha, L.C. Chan. (2000) Complex Cytogenetic Abnormalities in T-lymphoblastic Lymphoma. Resolution by Spectral Karyotyping. Cancer Genet Cytogenet.118: 24-27.

We describe a case of T-lymphoblastic lymphoma (T-LBL) in a 13-year-old boy in which conventional cytogenetic analysis of lymph node tissue showed complex karyotypic aberrations.  The present report illustrates that SKY technology is useful in identifying subtle translocations and resolving complex karyotypic aberrations in neoplastic disorders.

  1. A. Nordgren, A.G. Sorensen, N. Tinggaard-Pedersen, E. Blennow, C. Larsson, S. Lagercrantz. (2000) New chromosomal breakpoints in non-Hodgkin's lymphomas revealed by spectral karyotyping and G-banding. Int J Mol Med 5: 485-492.

Chromosomal rearrangements in short term cultures from nine cases of non-Hodgkin's lymphomas (NHL) were characterized by G-banding, spectral karyotyping (SKY), and fluorescence in situ hybridization (FISH). Eight of the nine cases showed complex karyotypes with chromosomal aberrations which, in most cases, could not be fully characterized by traditional G-banding analysis alone.  SKY and FISH analysis, as a complement to banding analysis, significantly improved the karyotypes in seven of the nine cases and unveiled 21 previously unidentified rearrangements with novel translocation breakpoints.

  1. T. Veldman, C. Vignon, E. Schröck, J.D. Rowley, T. Ried. (1997) Hidden chromosome abnormalities in haematological malignancies detected by multicolour spectral karyotyping. Nature Genetics 15: 406-410.

In 15 cases of hematological malignancies with complex chromosomal rearrangements, SKY analysis resulted in the elucidation of previously unidentified chromosomal material, as well as confirming all of the numerical chromosomal aberrations detected by G-banding analyses.

 

 


B) SOLID TUMORS

  1. A. Adeyinka, S. Kytola, F. Mertens, N. Pandis, C. Larsson. (2000) Spectral Karyotyping and chromosome banding studies of primary breast carcinomas and their lymph node metastases. Int. J Mol Med. March 5 (3): 235-240.

Three primary breast tumors and their lymph node metastases were characterized by G-banding, spectral karyotyping (SKY), and fluorescence in situ hybridization (FISH). In each case, the karyotypic abnormalities detected were similar in the primary tumor and its matched metastasis.  SKY and FISH confirmed the karyotypic similarities between the primary tumors and their metastases and, in addition, improved the identification and characterization of marker chromosomes.  The present study underscores the need to combine conventional chromosome banding and molecular cytogenetic techniques in the cytogenetic analysis of solid tumors.

  1. I.J. Cohen, J. Issakov, S. Avigad, B. Stark, I. Meller, R. Zaizov, I. Bar-Am. (1997) Synovial sarcoma of bone delineated by spectral karyotyping. The Lancet 350:1679-1680.

Spectral karyotyping permits rapid identification of complex and subtle chromosomal rearrangements in tumor cells without any prior knowledge of the chromosomes involved. We report a 22-year oldman with a 3-month history of pain in his left knee. All finding were consistent with a primitive neuroectodermal variant of Ewing sarcoma. Conventional  chromosome analysis showed a karyotype of 45 chromosomes with an unbalanced translocation from chromosome 22 to the short arm of chromosome 1. SKY unequivocally confirmed the presence of der(1)t(1;22) and in addition, detected a balanced translocation t(X;18), typical of synovial Sarcoma.

  1. M. Barnard , J Bayani , R. Grant R,  I.Teshima , P. Thorner , J.  Squire (2000).  Use of multicolor spectral karyotyping in genetic analysis of pleuropulmonary blastoma.
     Pediatr Dev Pathol Sep-Oct 3:5 479-86

Molecular analysis of the commonly encountered fusion translocation gene products of pediatric solid tumors failed to detect a rearrangement. Cytogenetic analysis, supplemented by multicolor spectral karyotyping (SKY), identified an unbalanced translocation between chromosomes 1 and X, resulting in additional copies of 1q, an extra copy of Xq, and loss of part of Xp. In addition, trisomy 8 was detected. The identification of new chromosomal alterations and confirmation of previously reported ones in this rare neoplasm helps to improve our understanding of its pathogenesis and association with other pediatric tumors.
.

  1. J. Bayani J, M Zielenska M, P. Marrano P, Ng. Y. Kwan  M.D. Taylor ,  V Jay ,   J.T. Rutka, J.A. Squire. (2000)  Molecular cytogenetic analysis of medulloblastomas and supratentorial primitive neuroectodermal tumors by using conventional banding, comparative genomic hybridization, and spectral karyotyping.J Neurosurg 2000 Sep 93:3 437-48

The CGH data demonstrate gains of chromosomes 17q and 7 in 60% of the tumors studied, which confirms data reported in the current literature. However, the authors have also combined the results of all three molecular cytogenetic assays (Giemsa banding, CGH, and SKY) to reveal the frequency of chromosomal rearrangement (gained, lost, or involved in structural rearrangement). CONCLUSIONS: The combined results indicate that chromosomes 7 and 17 are the most frequently rearranged chromosomes (10.1% and 8.9%, respectively, in all rearrangements detected). Furthermore, chromosomes 3 (7.8%), 14 (7%), 10 (6.7%), and 22 (6.5%) were also found to be frequently rearranged, followed by chromosomes 6 (6.5%), 13 (6.2%), and 18 (6.2%). Eight (33%) of 24 tumors exhibited high-level gains or gene amplification

  1. L. Trakhtenbrot, N. Cohen, E. Rosner, N. Gipsh, F. Brok-Simoni, M. Mandel, N. Amariglio, G. Rechavi. (1999) Coexistence of several unbalanced translocations in a case of neuroblastoma: The contribution of multicolor spectral karyotyping. Cancer Genet. Cytogenet. 112: 119-123.

Automatic classification, based on the measurement of the spectrum for each chromosome, was applied to metaphases obtained from the affected bone marrow of a neuroblastoma case. Spectral karyotyping allowed the identification of chromosomal aberrations that could not be identified by the use of the G-banding technique, and revealed a number of gains and unbalanced translocations.

 

 


C) CLINICAL CASES

  1. B. Huang, Y. Ning, A.N. Lamb, C.J. Sandlin, M. Jamehdor, T. Ried, J. Bartley. (1998) Identification of an unusual marker chromosome by spectral karyotyping. Am. J. Med. Genet. 80: 368-372.

We ascertained a newborn girl with multiple congenital anomalies including severe hypotonia, cardiovascular defects, hearing loss, central nervous system anomalies, and facial anomalies. The infant died at 12 days. Cytogenetic analysis showed a de novo supernumerary marker chromosome. Fluorescence in situ hybridization (FISH) with a combination of chromosome specific alpha-satellite probes and an all-human centromere probe failed to show hybridization to the marker, indicating that the marker chromosome lacked detectable alpha satellite sequences. Spectral karyotyping (SKY) was performed and showed that the marker was chromosome 15 in origin.

  1. Y.S. Fan, V.M. Siu, J.H. Jung, J. Xu. (2000) Sensitivity of multiple color spectral karyotyping in detecting small interchromosomal rearrangements. Genetic Testing 4: 9-14.

The sensitivity of SKY in detecting interchromosomal alterations was assessed with 10 constitutional translocations involving subtelomeric regions. Among the 13 small segments tested, 9 were clearly visualized and 8 were unambiguously identified by SKY. Fluorescence in situ hybridizations (FISH) with subtelomeric probes confirmed the reciprocity in three of the four translocations in which a small segment was not detectable by SKY. On the basis of resolution level of G-banding and the information obtained from the FISH analysis, the minimum alteration that SKY can detect is estimated to be 1,000-2,000 kbp in size with the currently available probes. This study has demonstrated the power, but also the limitations, of SKY in detecting small interchromosomal alterations, particularly those in subtelomeric regions.

  1. S.H. Morelli, D.A. Deubler, L.J. Brothman, J.C. Carey, A.R. Brothman. (1999) Partial trisomy 17p detected by spectral karyotyping. Clin Genet 55: 372-5.

We report the case of a child with partial trisomy of the short arm of chromosome 17, which was characterized by 24-color spectral karyotyping (SKY) and other fluorescence in situ hybridization (FISH) methods. The child had phenotypic features previously associated with trisomy 17p, including facial characteristics, developmental delay, postnatal growth retardation, single transverse crease, inguinal hernia, redundant neck skin folds, congenital heart defect, and club foot. This case illustrates the power of SKY for characterizing derivative/marker chromosomes in patients with rare cytogenetic syndromes.

  1. Y. Ning, C.H. Laundon, E. Schröck, P. Buchanan, T. Ried. (1999) Prenatal diagnosis of a mosaic extra structurally abnormal chromosome by spectral karyotyping. Prenat Diagn. May; 19(5):480-2.

A de novo mosaic extra structurally abnormal chromosome (ESAC) was detected in 33 per cent of cultured amniotic fluid cells from a pregnant woman. Neither Q-banding nor fluorescence in situ hybridization (FISH) employing a DNA probe for nucleolar organizer region demonstrated the presence of satellites on the ESAC. Spectral karyotyping (SKY) was performed in this prenatal case and led to a quick and accurate determination of the ESAC as chromosome 14 in origin.

  1. B. Peschka, J. Leygraaf, D. Hansmann, M. Hansmann, E. Schröck, T. Ried, H. Engels, G. Schwanitz, R. Schubert. (1999) Analysis of a de novo complex chromosome rearrangement involving chromosomes 4, 11, 12 and 13 and eight breakpoints by conventional cytogenetic, fluorescence in situ hybridization and spectral karyotyping. Prenat Diagn. December; 19(12): 1143-1149.

A complex chromosome rearrangement (CCR) with eight breakpoints resulting in four derivative chromosomes (4, 11, 12 and 13) was detected prenatally in a male fetus of a twin pregnancy. The karyotype of the female second fetus was normal. The apparently balanced de novo CCR was identified by classical cytogenetic methods and fluorescence in situ hybridization (FISH). We compared these findings with results from spectral karyotyping (SKY).

  1. M.C. Phelan, W. Blackburn, R.C. Rogers, E.C. Crawford, H.R. Cooley Jr., E. Schröck, Y. Ning, T. Ried. (1998) FISH analysis of a complex chromosome rearrangement involving nine breakpoints on chromosomes 6, 12, 14 and 16. Prenat. Diagn. 18: 1174-80.

We report the prenatal diagnosis of an apparently balanced de novo complex chromosome rearrangement (CCR) which involved nine breakpoints on four different chromosomes. Fluorescence in situ hybridization (FISH) and spectral karyotyping (SKY) were performed as an adjunct to G-banding for characterization of the abnormal chromosomes. The 22-week female fetus showed minor dysmorphic features including dolichocephaly, broad fingernails, tibial bowing, clubfoot, thoracolumbar scoliosis and hypoplastic toenails. Autopsy revealed gall-bladder hypoplasia and an atrial septal defect. Chromosome analysis of fetal tissue confirmed the presence of the complex rearrangement.

  1.  B.R. Haddad, E Shrock, J Meck, J Cowan, Young H, M.A. Ferguson Smith, S Du Manoir, T Ried (1998) Identification of de novo chromosomal markers and derivatives by spectral karyotyping. Hum Genet 103: 619-25

The purpose of this report is to demonstrate the application of SKY in the characterization of these de novo structural chromosomal abnormalities. Eight cases are described in this report. SKY has considerable diagnostic applications in prenatal diagnosis because of its reliability and speed. The identification of the chromosomal origin of markers and unbalanced translocations provides the patient, physician, and genetic counselor with better predictive information on the phenotype of the carrier.

  1. K.S. Reddy, V. Sulcova, H. Young, J.K. Blancato, B.R. Haddad. (1999) De novo mosaic add(3) characterized to betrisomy 14q31-qter using spectral karyotyping and subtelomeric probes . Am J Med Genet Feb 12;82(4):318-21

We describe a 19-year-old patient with a de novo mosaic add(3) chromosome (extra material of unknown origin on the 3q). The use of spectral karyotyping and fluorescence in situ hybridization using subtelomeric probes permitted the full characterization of the cytogenetic abnormality. The additional material on 3q was found to originate from 14q31-qter. This is one of the few reported cases with trisomy 14q31-qter and is the first mosaic case.

  1. E. Schröck, T. Veldman, H. Padilla-Nash, Y. Ning, J. Spurbeck, S. Jalal, L.G. Schaffer, P. Papenhausen, C. Kozma, M.C. Phelan, E. Kijeldsen, S.A. Schonberg, L. Biesecker, S. du Manoir, T. Ried. (1997) Spectral karyotyping refines cytogenetic diagnostics of constitutional chromosomal abnormalities. Hum. Genet. 101: 255-262.

Here, we report the comprehensive karyotype analysis of 16 samples from         different cytogenetic laboratories by merging conventional cytogenetic methodology and spectral karyotyping. This approach could become a powerful tool for the cytogeneticists, because it results in a considerable improvement of karyotype analysis by identifying chromosomal aberrations not previously detected by G-banding alone. Advantages, limitations, and future directions of spectral karyotyping are discussed.

 
 
 

D) CELL LINES AND GENERAL CYTOGENETICS
  1. R.J. Allen, S.D. Smith, R.L. Moldwin, M.M. Lu, L. Giordano, C. Vignon, Y. Suto, A. Harden, R. Tomek, T. Veldman, T. Ried, R.A. Larson, M.M. Le Beau, J.D. Rowley, N. Zeleznik-Le. (1998) Establishment and characterization of a megakaryoblast cell line with amplification of MLL. Leukemia 12: 1119-1127
  2. Y. Ariyama, T. Sakabe, T. Shinomiya, T. Mori, Y. Fukuda, J. Inazawa. (1998) Identification of amplified DNA sequences on double minute chromosomes in a leukemic cell line KY821 by means of spectral karyotyping and comparative genomic hybridization. J. Hum. Genet. 43: 187-190. .
  3. B.M. Ghadimi, E. Schröck, R.L. Walker, D. Wangsa, A. Jauho, P.S. Meltzer, T. Ried. (1999) Specific chromosomal aberrations and amplification of the AIB1 nuclear receptor coactivator gene in pancreatic carcinomas. Am. J. Pathol. 154: 525-36.
  4. J.D. Jiang, Y. Wang, C.A. Janish, J.F. Holland, J.G. Bekesi. (1998) 3-Bromoacetylamino benzoylurea (3-BAABU), a new antimicrotubule cancericidal agent applied in cytogenetic analysis in hematology. Biomed. Pharmacother. 52: 270-81.
  5. T. Knutsen, V.K. Rao, T. Ried, L. Mickley, E. Schneider, K. Miyake, B.M. Ghadimi, H. Padilla-Nash, S. Pack, L. Greenberger, K. Cowan, M. Dean, T. Fojo, S. Bates. (2000) Amplification of 4q21-q22 and the MXR gene in independently derived mitoxantrone-resistant cell lines. Genes Chromosomes Cancer 27: 110-116.
  6. N. Komae, Y. Hibino, N. Sugano (1999) Analysis of micronuclei induced under hyperthermic conditions in human lymphocyte culture by fluorescence in situ hybridization (FISH) and spectral karyotyping (SKY) methods. Yakugaku Zasshi 119: 763-772.
  7. S. Kytola, J. Rummukainen, A. Nordgren, R. Karhu, F. Farnebo, J. Isola, C. Larsson. Chromosomal alterations in 15 breast cancer cell lines by comparative genomic hybridization and spectral karyotyping. Genes, Chromosomes and Cancer 28: 308-317.
  8. J. Liang, Y. Ning, R-y Wang, H.M. Padilla-Nash, E. Schröck, D. Soenksen, L. Nagarajan, T. Ried. (1999) Spectral karyotypic study of the HL-60 cell line: detection of complex rearrangements involving chromosomes 5, 7, and 16 and delineation of critical region of deletion on 5q31.1 Cancer Genet. Cytogenet. 113: 105-109.
  9. W.O. Lui, S. Kytola, L. Anfalk, C. Larsson, L.O. Farnebo. (2000) Balanced Translocation (3;7)(p25;q34): Another Mechanism of Tumorigenesis in Follicular Thyroid Carcinoma. Cancer Genetics and Cytogenetics, Vol. 119:109-112.
  10. J.A. Macoska, B. Beheshti, J.S. Rhim, B. Hukku, J. Lehr, K.J. Pienta, J.A. Squire. (2000) Genetic characterization of immortalized human prostate epithelial cell cultures: evidence for structural rearrangements of chromosome 8 and i(8q) chromosome formation in malignant-derived cells . Cytogenet. Cell Genet. in press.
  11. M. Macville, E. Schröck, H. Padilla-Nash, C. Keck, B.M. Ghadini, D. Zimonjic, N. Popescu, T. Ried. (1999) Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping. Cancer Res. 59: 141-150.
  12. C. Marquez, J. Cohen, S. Munne. (1998) Chromosome identification in human oocytes and polar bodies by spectral karyotyping. Cytogenet. Cell Genet. 81: 254-258.
  13. R. Melcher, C. Steinlein, W. Feichtinger, C.R. Müller, T. Menzel, H. Lührs, W. Scheppach, M. Schmid. (2000) Spectral karyotyping of the human colon cancer cell lines SW480 and SW620. Cytogenet Cell Genet 88: 145–152.
  14. H.M. Padilla-Nash, W.G. Nash, K.M. Roberson, C.N. Robertson, M. Macville, E. Schröck, T. Ried. (1999) Molecular cytogenetic analysis of the bladder carcinoma cell line BK-10 by spectral karyotyping. Genes, Chromosomes & Cancer 25: 53-59.
  15. Y. Pan, S. Kytola, F. Farnebo, N. Wang, W.O. Lui, N. Nupponen, J. Isola, T. Visakorpi, U.S. Bergerheim, C. Larsson. (1999) Characterization of chromosomal abnormalities in prostate cancer cell lines by spectral karyotyping. Cytogenet Cell Genet. 87: 225-232.
  16. T. Satoh, K. Yamamoto, K.F. Miura, M. Jr. Ishidato. (1998) ICytogenetic analysis of heteromorphic short arm of 15p+ in a human diploid cell strain, TIG-7. Chromosome Science 2: 57-62.
  17. E. Schröck, S. du Manoir, T. Veldman, B. Schell, J. Weinberg, M.A. Ferguson-Smith, Y. Ning, D.H. Ledbetter, I. Bar-Am, D. Soenksen, Y. Garini, T. Ried. (1996) Multicolor spectral karyotyping of human chromosomes. Science 273: 494-497. (July 26, 1996).
  18. H. Vaziri, J.A. Squire, T.K. Pandita, G. Bradley, R.M. Kuba, H. Zhang, S. Gulyas, R.P. Hill, G.P. Nolan, S. Benchimol. (1999) Analysis of genomic integrity and p53-dependent G1 checkpoint in telomerase-induced extended-life-span human fibroblasts. Mol Cell Biol Mar:19(3):2373-9.
  19. S. Willadsen, J. Levron, S. Munne, T. Schimmel, C. Marquez, R. Scott, J. Cohen. (1999) Rapid visualization of metaphase chromosomes in single human blastomeres after fusion with in-vitro matured bovine eggs. Hum. Reprod. 14: 470-5.
  20. K.C. Bible, S.A. Boerner, K. Kirkland, K.L. Anderl, D. Jr. Bartelt, P.A. Svingen, T.J. Kottke, Y.K. Lee, S. Eckdahl, P.G. Stalboerger, R.B. Jenkins, SH. Kaufmann. (2000)Characterization of an ovarian carcinoma cell line resistant to cisplatin and flavopiridol. Clinical Cancer Res. 6:661-670.
  21. B. Beheshti, J. Karaskova, P.C. Park, J.A. Squire, B.G. Beatty. (2000) Identification of a high frequency of chromosomal rearrangements in the centromeric regions of prostate cancer cell lines by sequential Giemsa-banding and spectral karyotyping. Molecular Diagnosis 5: 23-32.

 
 

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..... to  HISKY

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