PathVysion^® HER-2 DNA Assay Filter Sets
The following filter sets are recommended for viewing and enumeration of the PathVysion^® HER-2 Assay. These filter sets are optimized both for Vysis SpectrumGreen^TM and SpectrumOrange^TM fluorophores and for paraffin-embedded specimen autofluorescence. The dual bandpass filter sets allow the user to view signals of each respective individual color fluorophore and the DAPI counterstain. The triple bandpass filter set allows the user to visualize the SpectrumGreen, SpectrumOrange, and DAPI fluorescent signals  simultaneously.

Vysis Filter Set	Fluorophores Detected
DAPI/9- Orange (NB) dual bandpass	SpectrumOrange and DAPI
DAPI/Green dual bandpass	SpectrumGreen and DAPI
DAPI/Green/Orange (V.2) triple bandpass	SpectrumGreen/SpectrumOrange/DAPI (specifically designed for viewing paraffin sections)

AneuVysion^® Assay Filter Sets
The following filter set configuration provides the best microscope filter set-up for viewing and analysis of the AneuVysion Assay on uncultured amniocytes.

Vysis Filter Set	Fluorophores Detected
Aqua single bandpass	SpectrumAquaTM
Green single bandpass	SpectrumGreenTM
Orange single bandpass	SpectrumOrangeTM
DAPI/Green/Orange triple bandpass	SpectrumGreen/SpectrumOrange/ DAPI

SpectraVysion^TM Filter Sets
Microscope filter sets for the SpectraVysion System are designed for optimum analysis of the fluorophores used in the SpectraVysion Assay and for analysis using the SpectraVysion Imaging System.

Vysis Filter Set	Fluorophores Detected
DAPI bandpass	DAPI
Aqua single bandpass	SpectrumAqua
Green single bandpass	SpectrumGreen
Red single bandpass	SpectrumRedTM
Gold single bandpass	SpectrumGoldTM
FRed (far red) single bandpass	SpectrumFRedTM

MultiVysion^® Assay Filter Sets
The following filter set configuration provides the optimum filter set-up for viewing and imaging the MultiVysion Assay result.

Vysis Filter Set	Fluorophores Detected
Aqua single bandpass	SpectrumAqua
Blue single bandpass	SpectrumBlueTM
Green single bandpass	SpectrumGreen
Yellow single bandpass	SpectrumGold
Red single bandpass	SpectrumRed

If viewing only, without image analysis, the following filter sets are required:

Vysis Filter Set	Fluorophores Detected
Aqua/Blue dual bandpass filter sets	SpectrumAqua/SpectrumBlue
Red/Green dual bandpass filter sets	SpectrumRed/SpectrumGreen
Yellow single bandpass	SpectrumGold

UroVysion^TM Assay Filter Sets

Vysis Filter Set	Fluorophores Detected
DAPI single bandpass	DAPI
Aqua single bandpass	SpectrumAqua
Yellow single bandpass	SpectrumGold
Red/Green dual bandpass	SpectrumRed/SpectrumGreen

Vysis filter sets can be specified for most microscope types and models. Some of the most common filter holders and sliders and their dimensions are included in the following table.Vysis currently does not supply the new 8 position turret for the Olympus AX-70. These can be ordered directly from your microscope dealer and shipped to Vysis for filter mounting.

Microscope Model	Filter Size Exciter	Dichroic*	Emitter
Leica Aristoplan	20 mm 9.2 mm max thickness	19 x 28 mm or 20 x 28 mm	20 mm 6.0 mm max thickness
Leica DM-RB (also called DM, DMR, LB)	21.9 mm 9.95 mm max thickness	22 x 29 mm or 21 x 29 mm
Leica DMIRB (also called DMIR and LS) Note:Used in 8 position automated turret of DMR microscope	20 mm	22 x 27 mm 0.75 mm	20 mm
Leica Ploem or LaborLux	18 mm 7.5 mm max thickness	26 mm 0.80 mm max thickness	18 mm 5.0 mm max thickness
Nikon Standard	18 mm 7.5 mm max thickness	18 x 26 mm 4.1 mm	18 mm
Nikon Quadfluor (X2F-EFD3)	25 mm	24 x 34 mm cut corners	25 mm 3.5 mm max thickness
Olympus BH-2 (RFCA, DMB, DMG, BHA, B-LO918)	20mm 9.0 mm max thickness	19 x 28 mm or 18 x 26 mm	20 mm 2.4 mm max thickness or 19 mm 5.0 mm max thickness
Olympus B-Max, U-M510 or BX	25 mm 6.0 mm max thickness	38 x 26 mm 1.0 mm max thickness	25 mm 6.0 mm max thickness
Olympus IMT-2	20mm 5.0 mm max thickness	21 x 29 mm	20 mm 6.0 mm max thickness
Zeiss Axioline, AxioSkop, Axioplan, Axiophot, 1 FL, 2 FL, 3 FL, 4 FL, 5 FL, 6 FL Axioplan 2, Axiophot 2 (cubes turret-5 position)	25 mm 5.4 mm max thickness	25.5 x 36 mm	25 mm 5.0 mm max thickness
Zeiss Standard IM35, 2 FL, 46, 63, 01	18 mm 6.0 mm max thickness	22 mm 0.8 mm max thickness	18 mm 6.0 mm max thickness

*Dichroic filter thickness is indicated only when there is a maximum. The thickness should be less than 1.5 mm to minimize image shift.